Mesothelial cells in ascitic fluid
The associated tumor antigen 90K is known to possess properties similar cytokine in modulating the cellular immune system, where accessory cells are the main target of this molecule. In 67 patients with ovarian cancer with significant amounts of ascites, were immune-stimulating proteins 90K detected in all samples of ascitic fluid studied.
Mesothelial cells in ascitic fluid, Levels correlated with ascites over 90K s-IL-2R content. To illustrate the source of 90K protein in the ascites fluid, his release in vitro was studied in primary cultured normal human peritoneal mesothelial cells (HPMC). Peritoneal mesothelium was found to produce five times more than 90K in the cells of ovarian cancer. 90K protein output was significantly increased by treatment with IFN-gamma in both mesothelial and cancer cells of the ovary.
However, neither IL-1 beta or TNF-alpha treatment consistently reduced the secretion of 90K in either cell type, mesothelial cells in ascitic fluid